abberior STAR deepRED, Nanobody FluoTag®-X2 anti-Mouse IgG1, 500 µl (5 µM)

Item number: N2002-AbdeepRED-S

Description

abberior STAR deepRED expands the abberior dye portfolio into the near-infrared (NIR) spectral range. Optimized for confocal and STED microscopy, it delivers bright fluorescence with minimal background signal and high photostability and is compatible with 775 nm STED depletion lasers. The dye can be efficiently excited with lasers around 680 nm and represents an excellent alternative to dyes such as CF680R, Alexa Fluor® 680, Cy®5.5, DyLight® 680, IRDye® 680LT, etc. Together with abberior STAR BLUE, GREEN, ORANGE, and RED, it enables flexible five-color imaging across a wide spectral range.

Secondary single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes – known for their superior brightness and photostability – with secondary sdABs (FluoTag®-X2) from NanoTag Biotechnologies provides an exceptional solution for high-quality, high-resolution imaging.

FluoTag®-X2 sdABs, developed by NanoTag Biotechnologies, bind with high affinity and specificity to a distinct epitope on mouse IgG1. Due to the inherent symmetry of the target antibody, two secondary nanobodies will be able to bind each primary antibody. The secondary FluoTag®-X2 reagents will therefore target 4 fluorophores to each primary antibody. This product includes one abberior STAR RED FluoTag®-X2 anti-Mouse IgG1 vial as a lyophilized reagent. Before staining, dissolve the reagent in 500 µl of sterile 50% glycerol (v/v) in deionized water to obtain a concentration of 5 µM of the nanobody (10 µM dye). Please refer to our protocol for detailed instructions on the handling, storage, and staining procedure.