abberior STAR 580, NHS ester, 1 mg
Item number
ST580-0002-1MG
Description
abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Characteristic features of this dye are exceptional photostability and high fluorescent quantum yield of 90%. abberior STAR 580 can be very effectively excited with excitation wavelength between 550 - 600 nm. For STED microscopy, abberior STAR 580 can be used most efficiently with a STED laser wavelength between 750 - 800 nm. Our abberior STAR 580 can substitute dyes like Cy3, ATTO® 590 or Alexa Fluor® 568. abberior STAR 580 is the ideal partner for abberior STAR RED or abberior STAR 635P to obtain optimal 2 color STED results. Together with our abberior STAR ORANGE you get the ideal pair for FLIM experiments. Best results are obtained with freshly prepared samples.
The NHS ester is a widespread tool for conjugating a fluorescent dye to a protein or antibody. NHS ester can be used to label primary amines of proteins or amine-modified molecules. The resulting abberior conjugate will show a bright fluorescence and photostability. abberior offers protocols for labeling antibodies via amine-reactive groups.
Properties
Extinction Coefficient
85000
Fluorescence Lifetime
3.5
Correction Factor 260
0.17
Correction Factor 280
0.17
Derivative/Conjugate
NHS ester
Photophysical properties were measured for carboxylic acid in PBS pH 7.4.
