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abberior STAR 520L


Item number
ST520L

Description

abberior STAR 520L is a long Stokes-shift dye optimized for STED and confocal microscopy in the orange-red spectral range. It shows a Stokes-shift of approx. 90 nm. As all long Stokes-shift dyes, it shows broad excitation and emission band: It can be excited between 490 - 550 nm and emits between 600 - 720 nm. For STED microscopy, abberior STAR 520L can be most efficiently used with STED laser wavelengths between 750 - 800 nm. abberior STAR 520L is designed and tested for multicolor STED microscopy in combination with abberior STAR 580 and abberior STAR RED using a single STED wavelength (775 nm). The hydrophilic properties of abberior STAR 520L conjugates reduce unspecific binding of this dye and thus decrease undesired background fluorescence. Our abberior STAR 520L can substitute dyes like DY-520XL® or DY-521XL®. abberior STAR 520SXP has been renamed abberior STAR 520L. The structure of the dye has not changed. Best results are obtained with freshly prepared samples.

Properties

Absorption
Absorption
515
Extinction Coefficient
Extinction Coefficient
60000
Emission
Emission
612
Quantum Efficiency
Quantum Efficiency
5
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
1
Correction Factor 260
Correction Factor 260
0.52
Correction Factor 280
Correction Factor 280
0.23
Charge
Charge
-2

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.





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abberior offers different kinds of embedding media for fluorescence microscopy.

abberior MOUNT embedding media are aqueous embedding media with a refractive index in the range of 1.38. They are very convenient in terms of application. However, due to evaporation of contained water and polymerisation of the embedding medium, the refractive index will change over time.

abberior TDE mounting media are 2,2'-thiodiethanol based embedding media with refractive indices in the range of 1.408 - 1.518. For many applications abberior TDE mounting media are superior to aqueous embedding media. Especially for demanding applications as 4Pi microscopy, 3D STED microscopy or isoSTED microscopy, because the refractive index will stay constant over time.

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