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abberior STAR 440L


Item number
ST440L

Description

abberior STAR 440L is a long Stokes-shift dye optimized for STED and confocal microscopy in the blue-green spectral range. It shows a Stokes-shift of approx. 80 nm. As all long Stokes-shift dyes, it shows a broad excitation and emission band: It can be excited between 400 - 470 nm and emits between 490 - 550 nm. For STED microscopy, abberior STAR 440L can be most efficiently used with STED laser wavelengths between 590 - 610 nm. It is therefore well suited for 2 color (STED) imaging together with our abberior STAR GREEN or abberior STAR 488. The hydrophilic properties of abberior STAR 440L conjugates preclude unspecific binding of this dye and thus decrease undesired background fluorescence. Our abberior STAR 440L can substitute dyes like ATTO® 425 or BD Horizon® V500. abberior STAR 440SXP has been renamed abberior STAR 440L. The structure of the dye has not changed. Best results are obtained with freshly prepared samples.

Properties

Absorption
Absorption
432
Extinction Coefficient
Extinction Coefficient
33000
Emission
Emission
511
Quantum Efficiency
Quantum Efficiency
57
STED min.
STED min.
590
STED max.
STED max.
610
Fluorescence Lifetime
Fluorescence Lifetime
3.7
Correction Factor 260
Correction Factor 260
0.47
Correction Factor 280
Correction Factor 280
0.31
Charge
Charge
0

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.





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abberior offers different kinds of embedding media for fluorescence microscopy.

abberior MOUNT embedding media are aqueous embedding media with a refractive index in the range of 1.38. They are very convenient in terms of application. However, due to evaporation of contained water and polymerisation of the embedding medium, the refractive index will change over time.

abberior TDE mounting media are 2,2'-thiodiethanol based embedding media with refractive indices in the range of 1.408 - 1.518. For many applications abberior TDE mounting media are superior to aqueous embedding media. Especially for demanding applications as 4Pi microscopy, 3D STED microscopy or isoSTED microscopy, because the refractive index will stay constant over time.

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