Description
abberior LIVE 590 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED, a depletion wavelength between 750 - 800 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 590 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue.
Our abberior LIVE 590 SNAP ligands can be readily used to specifically label your SNAP-tag® fusion proteins of interest within living cells, on cell surfaces, or in vitro. All our bright and photostable abberior LIVE SNAP probes are modified by a benzylguanine substrate as functional group. This product contains 1 vial of abberior LIVE 590 SNAP as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type, tissue, and expression rate of the SNAP-tag® protein a working solution of 10 nM to 1 µM is advised.
This product is covered by one or more patents owned or controlled by New England Biolabs, Inc. (NEB), and is intended for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.
SNAP-tag® and CLIP-tag® are registered trademarks of New England Biolabs, Inc.
