Derivate/Conjugate
Unit
450,00 €

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abberior LIVE 560, tubulin, 50 µg


Item number
LV560-0141-50UG

Description

abberior LIVE 560 is a dye designed for STED and confocal microscopy in living cells. Its molecular structure renders the dye strongly fluorogenic and facilitates its ability to penetrate cell membranes. It has an absorption maximum at 561 nm and can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 650 - 775 nm is recommended. Conjugates of abberior LIVE 560 enable live-cell imaging of DNA molecules or tubulin cytoskeleton. Our abberior LIVE 560 is the ideal partner for abberior LIVE 610 to obtain optimal 2 color live-cell imaging results.

Cell permeable and highly specific fluorescent probe used to label tubulin in living cells. This bright and photostable tubulin probe is based on the microtubule binding drug Cabazitaxel. This product contains 1 vial of abberior LIVE 560 tubulin as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type or tissue a working solution of 10 nM to 1 µM is advised. Additionally, a vial containing 1 µmol verapamil is provided as an imaging reagent. 10 to 20 nmol verapamil in 1 ml staining solution increases the signal intensity significantly.

Properties

Absorption
Absorption
561
Extinction Coefficient
Extinction Coefficient
78000
Emission
Emission
584
Quantum Efficiency
Quantum Efficiency
50
STED min.
STED min.
650
STED max.
STED max.
775
Fluorescence Lifetime
Fluorescence Lifetime
2.3
Charge
Charge
0
Molecular Weight
Molecular Weight
1392.6
Derivative/Conjugate
Tubulin (Cabazitaxel)

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.