abberior LIVE 550, actin, 50 µg

Item number: LV550-0140-50UG

Description

abberior LIVE 550 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 650 - 775 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 550 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue. Our abberior LIVE 550 is the ideal partner for abberior LIVE 610 to obtain optimal 2 color live-cell STED and confocal results.

Cell permeable and photostable fluorescent probe used to label actin in fixed and living cells. This highly specific actin probe is based on Jasplakinolide. This product contains 1 vial of abberior LIVE 550 actin as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type or tissue a working solution of 1 nM to 100 nM is advised. Additionally, a vial containing 1 µmol verapamil is provided as an imaging reagent. 10 to 20 nmol verapamil in 1 ml staining solution increases the signal intensity significantly.