Derivate/Conjugate
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abberior LIVE 510, SNAP, 30 nmol


Item number
LV510-0145-30NMOL

Description

abberior LIVE 510 is a dye designed for live-cell STED and confocal microscopy. Its molecular structure renders the dye strongly fluorogenic and facilitates its ability to overcome cell membranes. It can be optimally excited between 485 - 500 nm. For STED, a depletion wavelength between 590 - 610 nm is recommended. Conjugates of abberior LIVE 510 enable live-cell labeling of proteins and other biological structures e.g. actin or tubulin cytoskeleton.

Our abberior LIVE 510 SNAP ligands can be readily used to specifically label your SNAP-tag® fusion proteins of interest within living cells, on cell surfaces, or in vitro. All our bright and photostable abberior LIVE SNAP probes are modified by a benzylguanine substrate as functional group. This product contains 1 vial of abberior LIVE 510 SNAP as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type, tissue, and expression rate of the SNAP-tag® protein a working solution of 10 nM to 1 µM is advised.

This product is covered by one or more patents owned or controlled by New England Biolabs, Inc. (NEB), and is intended for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.
SNAP-tag® and CLIP-tag® are registered trademarks of New England Biolabs, Inc.

Properties

Absorption
Absorption
498
Extinction Coefficient
Extinction Coefficient
53000
Emission
Emission
529
Quantum Efficiency
Quantum Efficiency
94
STED min.
STED min.
590
STED max.
STED max.
610
Fluorescence Lifetime
Fluorescence Lifetime
3.8
Correction Factor 260
Correction Factor 260
0.26
Correction Factor 280
Correction Factor 280
0.18
Charge
Charge
0
Molecular Weight
Molecular Weight
826.7
Derivative/Conjugate
SNAP (Benzylguanine)

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.