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abberior CAGE 635


Item number
CA635

Description

abberior CAGE 635 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent red dye. It can most effectively be excited from 620 to 645 nm. abberior CAGE 635 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, abberior CAGE 635 can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.

Properties

Absorption
Absorption
630
Extinction Coefficient
Extinction Coefficient
110000
Quantum Efficiency
Quantum Efficiency
75
Activation
Activation
405
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
3.5
Charge
Charge
0

Photophysical properties were measured for carboxylic acid in PBS pH 7.4. Values refer to the photoactivated state of the dye.





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