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abberior CAGE 590


Item number
CA590

Description

abberior CAGE 590 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent orange-red dye. It can most effectively be excited from 560 to 600 nm. abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, abberior CAGE 590 can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.

Properties

Absorption
Absorption
595
Extinction Coefficient
Extinction Coefficient
75000
Emission
Emission
615
Quantum Efficiency
Quantum Efficiency
90
Activation
Activation
405
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
4
Charge
Charge
0

Photophysical properties were measured for carboxylic acid in PBS pH 7.4. Values refer to the photoactivated state of the dye.





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