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abberior CAGE 552


Item number
CA552

Description

abberior CAGE 552 is a caged dye which is initially nonfluorescent and colorless substance which, upon photolysis at 360 - 440 nm, readily transforms into a red and highly fluorescent dye. It absorbs around 552 nm and emits around 575 nm. abberior CAGE 552 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. After uncaging it can be effectively used for STED with a depletion wavelength between 650 - 775 nm. High brightness and a very good photostability are key features of the uncaged form of this dye. It is the smallest and lightest caged rhodamine dye available. Further, after photoactivation, abberior CAGE 552 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities in tracking experiments (in which the fluorescence signal is observed over time after the initial release of the fluorophore).

Properties

Absorption
Absorption
552
Extinction Coefficient
Extinction Coefficient
66000
Emission
Emission
574
Quantum Efficiency
Quantum Efficiency
73
Activation
Activation
405
STED min.
STED min.
650
STED max.
STED max.
775
Fluorescence Lifetime
Fluorescence Lifetime
4
Charge
Charge
0

Photophysical properties were measured for carboxylic acid in PBS pH 7.4. Values refer to the photoactivated state of the dye.





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