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abberior CAGE 500


Item number
CA500

Description

abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which has a fluoresces maximum at 525 nm. abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities in tracking experiments (in which the fluorescence signal is observed over time after the initial release of the fluorophore).

Properties

Absorption
Absorption
511
Extinction Coefficient
Extinction Coefficient
50000
Emission
Emission
525
Quantum Efficiency
Quantum Efficiency
85
Activation
Activation
405
STED min.
STED min.
590
STED max.
STED max.
610
Fluorescence Lifetime
Fluorescence Lifetime
4.1
Charge
Charge
0

Photophysical properties were measured for carboxylic acid in PBS pH 7.4. Values refer to the photoactivated state of the dye.





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