Derivative/Conjugate
Unit
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abberior STAR RED, nanobody FluoTag®-X2 anti-Rabbit IgG, 500 µL (5 µM)


Item number
N2402-AbRED-S

Description

abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. abberior STAR RED can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended.abberior STAR RED can substitute dyes like ATTO® 647N, Alexa Fluor® 647, or Cy5®. Our abberior STAR RED is the ideal partner for abberior STAR ORANGE or abberior STAR 580 to obtain optimal 2 color STED results. Best results are obtained with freshly prepared samples.

NEW: Learn more about our latest variants conjugated to Smart Secondaries® (nanobodies) optimized for efficient labeling and minimal linkage error in STED and confocal microscopy.



Secondary single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes – known for their superior brightness and photostability – with secondary sdABs (FluoTag®-X2) from NanoTag Biotechnologies provides an exceptional solution for high-quality, high-resolution imaging. FluoTag®-X2 sdABs, developed by NanoTag Biotechnologies, bind with high affinity and specificity to a distinct epitope on rabbit IgG. Due to the inherent symmetry of the target antibody, two secondary nanobodies will be able to bind each primary antibody. The secondary FluoTag®-X2 reagents will therefore target 4 fluorophores to each primary antibody. This product includes one abberior STAR RED FluoTag®-X2 anti-Rabbit IgG vial as a lyophilized reagent. Before staining, dissolve the reagent in 500 µl of sterile 50% glycerol (v/v) in deionized water to obtain a concentration of 5 µM of the nanobody (10 µM dye). Please refer to our protocol for detailed instructions on handling, storage, and staining procedure

Properties

Absorption
Absorption
638
Extinction Coefficient
Extinction Coefficient
120000
Emission
Emission
655
Quantum Efficiency
Quantum Efficiency
90
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
3.4
Correction Factor 260
Correction Factor 260
0.16
Correction Factor 280
Correction Factor 280
0.32
Charge
Charge
-1
Derivative/Conjugate
Nanobody anti-rabbit, monoclonal

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.