Derivative/Conjugate
Unit
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abberior STAR RED, membrane, 5 nmol


Item number
STRED-0206-5NMOL

Description

abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. abberior STAR RED can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended.abberior STAR RED can substitute dyes like ATTO® 647N, Alexa Fluor® 647, or Cy5®. Our abberior STAR RED is the ideal partner for abberior STAR ORANGE or abberior STAR 580 to obtain optimal 2 color STED results. Best results are obtained with freshly prepared samples.

Our abberior STAR RED membrane probes can be readily used to specifically label the outer plasma membrane of living cells or can be used for in vitro studies. All our bright and photostable abberior STAR membrane probes are modified by cholesterol as a functional group. Since the dye is not incorporated into the membrane, our unique abberior membrane probes do not change the composition of the plasma membrane by foreign substances. The natural behavior of the membrane and fluidity will maintain. This product contains 1 vial of abberior STAR RED membrane as a lyophilized powder, a stock solution of 10 µM in DMF or DMSO is recommended. Depending on cell type, or tissue a working solution of 10 to 50 nM in aqueous media is advised, which is sufficient for up to 1000 cell stainings.

Properties

Absorption
Absorption
638
Extinction Coefficient
Extinction Coefficient
120000
Emission
Emission
655
Quantum Efficiency
Quantum Efficiency
90
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
3.4
Correction Factor 260
Correction Factor 260
0.16
Correction Factor 280
Correction Factor 280
0.32
Charge
Charge
-1
Molecular Weight
Molecular Weight
3381.1
Derivative/Conjugate
Membrane (Cholesterol)

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.