Description
abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. abberior STAR RED can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended.abberior STAR RED can substitute dyes like ATTO® 647N, Alexa Fluor® 647, or Cy5®. Our abberior STAR RED is the ideal partner for abberior STAR ORANGE or abberior STAR 580 to obtain optimal 2 color STED results. Best results are obtained with freshly prepared samples.
NEW: Learn more about our latest variants conjugated to Smart Secondaries® (nanobodies) optimized for efficient labeling and minimal linkage error in STED and confocal microscopy.
Single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes—known for their superior brightness and photostability—and FluoTag®-X4 anti-GFP from NanoTag provides an exceptional solution for high-quality, high-resolution imaging.
FluoTag®-X4 anti-GFP, developed by NanoTag, is a unique blend of two sdABs that bind with high affinity and specificity to two distinct epitopes on GFP and its most common derivatives. This enables direct and highly specific fluorescent detection of GFP-tagged targets, eliminating the need for secondary antibodies. This product includes one abberior STAR RED FluoTag®-X4 anti-GFP vial as a lyophilized reagent. FluoTag®-X4 anti-GFP is a carefully optimized blend of two high-affinity, monovalent single-domain antibody (sdAb) clones (#1H1 and #1B2), each targeting a distinct epitope on GFP. Before staining, dissolve the reagent in 200 µl of sterile 50% glycerol (v/v) in deionized water to obtain a concentration of 5 µM dye, 1.25 µM of each sdAb. Please refer to our protocol for detailed instructions on the staining procedure, handling, and storage.
