Derivative/Conjugate
Unit
350,00 €

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abberior STAR ORANGE, membrane, 5 nmol


Item number
STORANGE-0206-5NMOL

Description

abberior STAR ORANGE is a novel fluorescent dye developed for STED and confocal microscopy in the orange spectral region. Introducing negatively charged groups into its molecular structure making the dye excellently water soluble and enables background-free imaging. Thus, abberior STAR ORANGE is highly photostable and a bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED microscopy, this dye can be most efficiently used with a STED laser wavelength between 750 - 800 nm. Our abberior STAR ORANGE can substitute dyes like ATTO® 594 or Alexa Fluor® 594. Together with our abberior STAR RED you can obtain stunning 2 color STED results. In combination with our abberior STAR 580 you get the ideal pair for FLIM experiments. Best results are obtained with freshly prepared samples.

Our abberior STAR ORANGE membrane probes can be readily used to specifically label the outer plasma membrane of living cells or can be used for in vitro studies. All our bright and photostable abberior STAR membrane probes are modified by cholesterol as a functional group. Since the dye is not incorporated into the membrane, our unique abberior membrane probes do not change the composition of the plasma membrane by foreign substances. The natural behavior of the membrane and fluidity will maintain. This product contains 1 vial of abberior STAR ORANGE membrane as a lyophilized powder, a stock solution of 10 µM in DMF or DMSO is recommended. Depending on cell type, or tissue a working solution of 10 to 50 nM in aqueous media is advised, which is sufficient for up to 1000 cell stainings.

Properties

Absorption
Absorption
589
Extinction Coefficient
Extinction Coefficient
95000
Emission
Emission
616
Quantum Efficiency
Quantum Efficiency
55
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
4.5
Correction Factor 260
Correction Factor 260
0.55
Correction Factor 280
Correction Factor 280
0.56
Charge
Charge
-3
Molecular Weight
Molecular Weight
3289.9
Derivative/Conjugate
Membrane (Cholesterol)

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.





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