abberior STAR 580, nanobody anti-mouse IgG, 50 µl (1 mg/ml)
Item number
ST580-1009-50UG
Description
abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Characteristic features of this dye are exceptional photostability and high fluorescent quantum yield of 90%. abberior STAR 580 can be very effectively excited with excitation wavelength between 550 - 600 nm. For STED microscopy, abberior STAR 580 can be used most efficiently with a STED laser wavelength between 750 - 800 nm. Our abberior STAR 580 can substitute dyes like Cy3, ATTO® 590 or Alexa Fluor® 568. abberior STAR 580 is the ideal partner for abberior STAR RED or abberior STAR 635P to obtain optimal 2 color STED results. Together with our abberior STAR ORANGE you get the ideal pair for FLIM experiments. Best results are obtained with freshly prepared samples.
Nanobodies are an exciting, novel antibody format. Being 10x smaller than conventional whole IgG antibodies, the 15kDa nanobodies are perfect for high resolution imaging experiments where small labels, good penetration and outstanding specificity is necessary. The combination of super bright and highly photostable abberior STAR dyes and the polyclonal nanobodies produced in alpacas from Jackson ImmunoResearch (AffiniPure-VHH™ Secondaries) offer a fantastic solution for high-quality and high-resolution imaging.
The nanobodies are cross-adsorbed for exquisite specificity against target species with minimal cross-reactivity to other commonly used species, making them suitable for application in multiple labeling experiments.
This product contains 1 vial of abberior STAR 580 nanobody anti-mouse IgG as a solution with a concentration of 1 mg/ml.
More information regarding staining procedure, handling and storage will be find in our protocol.
Properties
Extinction Coefficient
85000
Fluorescence Lifetime
3.5
Correction Factor 260
0.17
Correction Factor 280
0.17
Derivative/Conjugate
Nanobody anti-mouse
Photophysical properties were measured for carboxylic acid in PBS pH 7.4.