abberior STAR 488, NHS ester, 1 mg
Item number
ST488-0002-1MG
Description
abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, this fluorescent dye can be efficiently used with STED laser wavelengths between 590 - 610 nm. Introducing negatively charged groups into its molecular structure making the dye excellent water soluble. Our abberior STAR 488 can substitute dyes like Oregon Green® 488, ATTO® 488 or Alexa Fluor® 488. Together with our abberior STAR 440SXP you can obtain optimal 2 color STED results with a single depletion laser. Best results are obtained with freshly prepared samples.
The NHS ester is a widespread tool for conjugating a fluorescent dye to a protein or antibody. NHS ester can be used to label primary amines of proteins or amine-modified molecules. The resulting abberior conjugate will show a bright fluorescence and photostability. abberior offers protocols for labeling antibodies via amine-reactive groups.
Properties
Extinction Coefficient
65000
Fluorescence Lifetime
3.9
Correction Factor 260
0.28
Correction Factor 280
0.14
Derivative/Conjugate
NHS ester
Photophysical properties were measured for carboxylic acid in PBS pH 7.4. Fluorescence Lifetime was measured in MeOH + 0.05% TFA.
