Description
abberior STAR 460L is a long Stokes-shift dye optimized for STED and confocal microscopy. It shows a large Stokes-shift of approx. 162 nm and has a broad excitation and emission band: The dye is excited most efficiently in the range of 440 - 490 nm and emits between 560 - 680 nm. For STED microscopy abberior STAR 460L can be used with STED laser wavelengths between 750 - 800 nm. All these features making abberior STAR 460L the optimal dye for multicolor STED experiments, in particular as 3rd color in combination with abberior STAR ORANGE and STAR RED.
NEW: Learn more about our latest variants conjugated to Smart Secondaries® (nanobodies) optimized for efficient labeling and minimal linkage error in STED and confocal microscopy.
Secondary single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes – known for their superior brightness and photostability – with secondary sdABs (FluoTag®-X2) from NanoTag Biotechnologies provides an exceptional solution for high-quality, high-resolution imaging.
FluoTag®-X2 sdABs, developed by NanoTag Biotechnologies, bind with high affinity and specificity to a distinct epitope on mouse IgG1. Due to the inherent symmetry of the target antibody, two secondary nanobodies will be able to bind each primary antibody. The secondary FluoTag®-X2 reagents will therefore target 4 fluorophores to each primary antibody. This product includes one abberior STAR 460L FluoTag®-X2 anti-Mouse IgG1 vial as a lyophilized reagent. Before staining, dissolve the reagent in 500 µl of sterile 50% glycerol (v/v) in deionized water to obtain a concentration of 5 µM of the nanobody (10 µM dye). Please refer to our protocol for detailed instructions on the handling, storage, and staining procedure.
