abberior LIVE ORANGE, mito, 300 stainings

Item number: LVORANGE-0146-300STN

Description

abberior LIVE ORANGE mito is a fluorescent probe that specifically stains the cristae in mitochondria. Now, in combination with STED microscopy, these fine mitochondrial components become visible and can be investigate using this organic fluorophore. Due to the chemical structure of the mito-probe, repeated excitation with light over a long period of time is possible without generating toxic effects. This creates the possibility to observe the movement of mitochondria and their substructures through microscopic imaging. The probe can be combined with other fluorescent probes from our abberior LIVE series. This probe shows bright orange fluorescence, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 750 - 800 nm is recommended.

LIVE ORANGE Mito is supplied as a lyophilized powder and should be reconstituted in 150 µL DMF or DMSO. The resulting stock solution can be diluted 1:1000 for use. At an assay volume of 500 µL, the product is sufficient for up to 300 stainings.

Note: Depending on the cell type and assay setup, optimization of the dilution factor may be required.