abberior LIVE 550, click, 30 nmol
abberior LIVE 550 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with a 561 nm laser. For STED, a depletion wavelength between 650 - 775 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 550 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue. Our abberior LIVE 550 is the ideal partner for abberior LIVE 610 to obtain optimal 2 color live-cell STED and confocal results.
All our bright and photostable abberior LIVE click probes are modified by a tetrazine substrate as a functional group. They react with dienophiles such as trans-cyclooctene (TCO) or bicyclononyne (BCN) derivatives. Along with this bioorthogonal reaction, LIVE click probes are particularly useful in combination with unnatural amino acid (uAA) with a TCO or BCN side chain. The incorporation of uAAs prepares site-selective protein conjugates with a very specific and minimum-sized tag. abberior LIVE click is optimal for super-resolution microscopy within living cells, on cell surfaces, or for in vitro applications. This product contains 1 vial of abberior LIVE 550 click as a lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type, tissue, and expression rate of the unnatural amino acid a working solution of 10 nM to 1 µM is advised.
Photophysical properties were measured for carboxylic acid in PBS pH 7.4.