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abberior LIVE 510, mito, 300 stainings

  • Item number: LV510-0146-300STN

Description

abberior LIVE 510 is a dye designed for live-cell STED and confocal microscopy. Its molecular structure renders the dye strongly fluorogenic and facilitates its ability to overcome cell membranes. It can be optimally excited between 485 - 500 nm. For STED, a depletion wavelength between 590 - 610 nm is recommended. Conjugates of abberior LIVE 510 enable live-cell labeling of proteins and other biological structures e.g. actin or tubulin cytoskeleton.

LIVE 510 Mito is supplied as a lyophilized powder and should be reconstituted in 150 µL DMF or DMSO. The resulting stock solution can be diluted 1:1000 for use. At an assay volume of 500 µL, the product is sufficient for up to 300 stainings.

Note: Depending on the cell type and assay setup, optimization of the dilution factor may be required.

Properties

Absorption
Absorption
498
Extinction Coefficient
Extinction Coefficient
53000
Emission
Emission
529
Quantum Efficiency
Quantum Efficiency
94
STED min.
STED min.
590
STED max.
STED max.
610
Fluorescence Lifetime
Fluorescence Lifetime
3.8
Correction Factor 260
Correction Factor 260
0.26
Correction Factor 280
Correction Factor 280
0.18
Charge
Charge
0
Molecular Weight
Molecular Weight
909.6
Derivative/Conjugate
mitochondria

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.

abberior LIVE 510

Derivate/Conjugate 
Unit 
395,00 €
excl. 19% VAT , plus shipping costs
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