abberior LIVE 510, actin, 50 µg
Item number
LV510-0140-50UG
Description
abberior LIVE 510 is a dye designed for live-cell STED and confocal microscopy. Its molecular structure renders the dye strongly fluorogenic and facilitates its ability to overcome cell membranes. It can be optimally excited between 485 - 500 nm. For STED, a depletion wavelength between 590 - 610 nm is recommended. Conjugates of abberior LIVE 510 enable live-cell labeling of proteins and other biological structures e.g. actin or tubulin cytoskeleton.
Cell permeable and photostable fluorescent probe used to label actin in fixed and living cells. This highly specific actin probe is based on Jasplakinolide. This product contains 1 vial of abberior LIVE 510 actin as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type or tissue a working solution of 10 nM to 1 µM is advised. Additionally, a vial containing 1 µmol verapamil is provided as an imaging reagent. 10 to 20 nmol verapamil in 1 ml staining solution increases the signal intensity significantly.
Properties
Extinction Coefficient
53000
Fluorescence Lifetime
3.8
Correction Factor 260
0.26
Correction Factor 280
0.18
Derivative/Conjugate
Actin (jasplakinolide)
Photophysical properties were measured for carboxylic acid in PBS pH 7.4.
