Description
abberior STAR 550 was developed for STED and confocal microscopy in the green-orange spectral region. Characteristic features of this dye are its exceptional photostability and brightness. abberior STAR 550 can be very effectively excited with a 518 nm laser. For STED microscopy, abberior STAR 550 can be used most efficiently with a STED laser wavelength between 650 – 775 nm. In combination with abberior STAR BLUE, abberior STAR ORANGE, and abberior STAR RED, abberior STAR 550 enables versatile 4-color imaging across a broad spectral range. Best results are obtained with freshly prepared samples.
Secondary single-domain antibodies (sdABs), also known as nanobodies, represent an innovative and promising antibody format. At just 15 kDa, sdABs are 10 times smaller than conventional whole IgG antibodies, making them ideal for high-resolution imaging applications that require small labels, excellent tissue penetration, and exceptional specificity. The combination of abberior STAR dyes – known for their superior brightness and photostability – with secondary sdABs (monoclonal FluoTag®-X2 secondary nanobodies or Smart Secondaries®) from NanoTag Biotechnologies provides an exceptional solution for high-quality, high-resolution imaging.
FluoTag®-X2 sdABs, developed by NanoTag Biotechnologies, bind with high affinity and specificity to a distinct epitope on rabbit IgG. Due to the inherent symmetry of the target antibody, two secondary nanobodies will be able to bind each primary antibody. The secondary FluoTag®-X2 reagents will therefore target 4 fluorophores to each primary antibody. This product includes one abberior STAR 550 FluoTag®-X2 anti-Rabbit IgG vial as a lyophilized reagent. Before staining, dissolve the reagent in 500 µl of sterile 50% glycerol (v/v) in deionized water to obtain a concentration of 5 µM of the nanobody (10 µM dye). Please refer to our protocol for detailed instructions on the handling, storage, and staining procedure.
