Derivative/Conjugate
Unit
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abberior STAR RED, nanobody anti-mouse IgG, 50 µl (1 mg/ml)


Item number
STRED-1009-50UG

Description

abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. abberior STAR RED can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended.abberior STAR RED can substitute dyes like ATTO® 647N, Alexa Fluor® 647, or Cy5®. Our abberior STAR RED is the ideal partner for abberior STAR ORANGE or abberior STAR 580 to obtain optimal 2 color STED results. Best results are obtained with freshly prepared samples.

Nanobodies are an exciting, novel antibody format. Being 10x smaller than conventional whole IgG antibodies, the 15kDa nanobodies are perfect for high resolution imaging experiments where small labels, good penetration and outstanding specificity is necessary. The combination of super bright and highly photostable abberior STAR dyes and the polyclonal nanobodies produced in alpacas from Jackson ImmunoResearch (AffiniPure-VHH™ Secondaries) offer a fantastic solution for high-quality and high-resolution imaging. The nanobodies are cross-adsorbed for exquisite specificity against target species with minimal cross-reactivity to other commonly used species, making them suitable for application in multiple labeling experiments. This product contains 1 vial of abberior STAR RED nanobody anti-mouse IgG as a solution with a concentration of 1 mg/ml. More information regarding staining procedure, handling and storage will be find in our protocol.

Properties

Absorption
Absorption
638
Extinction Coefficient
Extinction Coefficient
120000
Emission
Emission
655
Quantum Efficiency
Quantum Efficiency
90
STED min.
STED min.
750
STED max.
STED max.
800
Fluorescence Lifetime
Fluorescence Lifetime
3.4
Correction Factor 260
Correction Factor 260
0.16
Correction Factor 280
Correction Factor 280
0.32
Charge
Charge
-1
Derivative/Conjugate
Nanobody anti-mouse

Photophysical properties were measured for carboxylic acid in PBS pH 7.4.