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abberior CAGE 532


Item number
CA532

Description

abberior CAGE 532 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye. The dye is optimized for excitation light around 532 nm. abberior CAGE 532 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, abberior CAGE 532 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities in tracking experiments (in which the fluorescence signal is observed over time after the initial release of the fluorophore).

Properties

Absorption
Absorption
533
Extinction Coefficient
Extinction Coefficient
82000
Emission
Emission
541
Quantum Efficiency
Quantum Efficiency
84
Activation
Activation
405
STED min.
STED min.
590
STED max.
STED max.
610
Fluorescence Lifetime
Fluorescence Lifetime
4.1
Charge
Charge
0

Photophysical properties were measured for carboxylic acid in PBS pH 7.4. Values refer to the photoactivated state of the dye.





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